Nikon OPTIPHOT-POL Manual de instrucciones - Página 16

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Nikon OPTIPHOT-POL Manual de instrucciones

V.PHOTOMICROGRAPHY

Prepare the following

equipments in addition to

the OPTIPHOT-POL

microscope main body.

Nikon Microflex

Trinocular

eyepiece tube "TP"

CF PL Projection lens

1. CF PI:.Projection Lenses

The combined

use of the CF P objectives and

CF PL Projection lenses is essential.

For the same total magnification,

select a com-

bination

of the highest possible objective power

and lowest possible projection

lens power to

achieve

the

utmost

image

definition

and

contrast.

Illumination

1) Checking the illu mination

Unevenness in the illumination

will show

conspicuously

in photomicro-

graphy than in observation. Consequently,

before

taking

a photograph,

recheck the

positioning

and centering of the lamp and

the correct adjustment of the condenser.

2) Selection of voltage and filter

The color temperature

of the light source

varies with the voltage being used. There-

fore,

color

photomicrography,

the

selection

of voltage and filter

is essential

(for the result to be obtained).

Table 3.

Standard Selection

Film

Voltage

Filter

NCB 10 is to be used

type

film

Tungsten

type

Remove NCB 10

film

Over 6

Contrast fi Iter(green),

etc. is usable

Table 3 shows the standard combination.

Depending

upon the make of the film,

different

color renditions

may result. It is

recommended that in addition

to the NCB

10 filter

a color compensation

filter

(CC

filter),

available

from

the film

manufac-

tu rer, be used.

Shutter Speed

Desirable shutter speeds for least vibration

are

1/4 ~ 1/15 sec. Adjustment

of the image bright-

ness for

color

photomicrography

should

made by means of the NO filters.

4. Manipulation of Field and Aperture

Diaphragm

In photomicrography,

the adjustment

of the

field diaphragm is important

for the purpose of

limiting

extraneous

light which causes flare in

the

microscope

image. Stop

down

the dia-

phragm so as to get an illuminated

area slightly

larger than that of the picture field. By adjust-

ing the aperture diaphragm, a change of depth

of focus, contrast

and resolution

of image is

attainable.

Select a size suited to the purpose.

Generally speaking, the aperture diaphragm,

properly

stopped down to 70 ~ 80% of the

aperture of the objective being used.

5. Focusing

Focusing for photomicrography

can be done

with

the observation

tube

of the trinocular

eyepiece tube "TP"

or by using the Microflex

finder.

Adjust diopter

•

Using binocular of eyepiece tube:

Use

or 1

objective.

Insert the mask eyepiece into

either of

right

or left eyepiece sleeve that

is ac-

customed to usual use. Adjust the diopter

ring to bring the double cross line in the

view field center into focus. (Fig. 28)

Then focus the specimen image also on the

central area of the mask by means of the

focus knob of the microscope.

The diopter

of another eyepiece is to be

adjusted by focusing specimen rotating the

diopter

ring without

using the microscope

focus knob.

Rotate the mask eyepiece so as the mask

positions as shown in Fig. 32.