Amersham Pharmacia Biotech P-910 Petunjuk Manual - Halaman 3

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8 Measure the volume collected in

the measuring cylinder. Enter this value in

. Click on

Reference field

click on

Save

9 The pump is now calibrated.

Storage

Overnight: The pump can be left filled with a

buffer.

Weekend and Long time storage: Flush the

pump with water and then fill it with 20%

Ethanol.

Note: If the pump tubing is left in the pump

for a long period it will become deformed

and will not function correctly.

For long time storage remove the pump

tubing from the pump to avoid deformation

of the tubing.

Pump P-910

Instructions 56-3000-47 Edition AD

then

Set ref value

4 Maintenance

CAUTION! Only spare parts approved or

supplied by Amersham Pharmacia Biotech

may be used for maintaining and servicing

Pump P–910.

Period

Action

If tubing breaks

Perform cleaning procedure

detailed below.

Every 200 hrs

Replace pump tubing. See

section 2.

Cleaning-in-place

Pump a cleaning or sanitizing agent through

the pump. The standard recommendation is

to pump 1 M NaOH for 30 minutes and then

wash out with buffer.

WARNING! NaOH is injurious to health.

Avoid spillage.

Cleaning procedure

1 Open the door and remove the tubing.

2 Dismantle the roller cage by undoing

the Allen screw and lifting the cage

straight out. Take care not to lose the two

metal washers.

3 Slide the pressure plate forward and

remove it from the pump.

4 Clean the roller cage and the roller cage

housing with soapy water.

5 If the roller cage O-ring has come into

contact with the eluent, replace the O-ring.

6 Re-assemble the pump by reversing

the dismantling procedure.

5 Trouble shooting

If the suggested actions do not correct the

fault, call Amersham Pharmacia Biotech.

Fault

Action

Leakage

1 Check the top and bottom pump

tubing connections.

2 Check if there is damage to the

pump tubing. Replace if necessary.

Not running

Check the UniNet connections.