Nikon Eclipse Ti TIFT Ee-1454 Manual - Halaman 8

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MDA: Time lapse imaging

• Activate the time-lapse option in MDA's main window

• Set the desired interval and time

• Choose the desired illumination settings in the wavelength tab

o Or multiple when the option multiple wavelengths are selected

• Start recording by clicking on Acquire

Shut down system

• For live cell imaging:

o Remove Tokai Hit incubator from the stage and place it on the table

o Close the Tokai Hit incubator with a plastic well

• Clean objective carefully

o Remove oil with lens paper:

o Hold lens paper tight between your fingers and wipe over objective

o Clean lens with 2-propanol on your lens paper

• Cover stage with lens paper box

• Save data to OIC network storage (O:\ drive)

o To collect the data from your own pc, connect to network storage drive via the address:

\\oic-station\oic

user: guest, password: guest

o When the hard disk of the microscopes pc gets full, data will be deleted

o You are responsible for your own data, the networkdrive is only for transport

• If you are the last user of the day

• Shut down all devices

o Shut down mercury lamp as last

o Camera power is switched at the adapter box at the floor

• Switch off the power socket located at the floor

Contact

• Martijn de Gruiter

• Gert-Jan Kremers

Martijn de Gruiter

Be-343

tel. 31105

Be-346

tel. 43578

Manual: TIRF Microscope

Erasmus OIC

Ee-1454