AmScope AFDM1080 Manuale - Pagina 12

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Measurement Calibration

Before using measurement tools, the calibration process must be finished for the desired magnification. The purpose of

this process is to create a magnification profile which defines the relationship between camera pixels and real units of

measure such as millimeters. Once this relationship is defined, any shape drawn on the screen will provide accurate mea-

surements by translating the number of pixels into the desired unit of measure. Because the calibrated profile will only

be accurate for the magnification used during the calibration, any change to the microscope's zoom or working distance

will affect the accuracy of measurements.

To begin the process, click the calibration icon. A message will appear on the screen stating that the camera is resetting.

The zoom factor and focal point will reset to neutral positions. A message will appear on the screen instructing you to

place a reference object under the microscope, then adjusting the height of the microscope until the object is in clear

focus. A ruler with millimeter graduations is ideal for this process. You can adjust the zoom factor using the mouse's scroll

wheel. Once the reference object is in clear focus, click the OK button.

A line will appear on the screen along with a Calibration window. Before a measurement can be recorded, you must en-

ter a value in the Calibration window's Magnification field. The zoom factor which appears in the top-right corner of the

screen can be used (you may need to move the Calibration window to reveal the zoom factor). Once the value has been

entered, click the Apply button. Next, adjust the length of the calibration line so it aligns with the reference object, and

spans a measurable distance. It is recommended to us the longest practical distance, as this will yield the best accuracy.

Four buttons on the bottom of the screen can be used to nudge the line. Next, enter the measured length in the Actual

Length field, then select the appropriate units. The Monitor Size field is used to define the diagonal measurement of the

screen being used, so the monitor's magnification of the on-screen image is used in the calculation. Once all values have

been entered, click the OK button.

This calibrated magnification can now be accessed from the toolbar. You can select your preferred unit of measure, and

on-screen measurements will be calculated using that unit.

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