AmScope B270 Manual - Page 15

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2.3 Using the Condenser

The condenser focuses light from the microscope's lamp onto the specimen in order to maximize brightness and resolu-

tion. There are multiple adjustments which can be made to suit different applications.

2.3a Condenser Height

[image: adjusting condenser height]

The condenser should be raised to its maximum height to achieve optimal brightness and resolution. The height-adjust-

ment knob is located on the left side of the microscope, just behind the condenser holder. Rotate the knob clockwise to

lower the condenser, and counter-clockwise to raise the condenser. Lowering the condenser is typically done to perform

a function such as applying immersion oil. The height of the condenser also affects its numerical aperture. Lowering the

condenser will decrease the effective aperture, therefore increasing the observed depth of field in a manner similar to

using the iris diaphragm. This can negatively impact image quality due to diffraction, so it is recommended to use the

condenser at its maximum height.

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