AmScope SE400 Series User Manual - Page 12

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SE400 Series

Technical Terms & Concepts

Total Magnification

Total magnification of a microscope is calculated by the magnification of the objective multiplied by the mag-

nification of the eyepieces.

-Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification

Field of View

Linear field of view of the eyepiece divided by the magnification of the objective

Numerical Aperture (N.A)

Calculated by n Sin α (max), the Numerical Aperture (N.A) is an important parameter that marks the

features of the objective and condenser's image quality and resolution. The "n" is a refractive index of the medium

(air or immersion cedar oil) between the objective lens and the specimen. The "α" is 1/2 of the angle between the

aperture on the objective and path of light. The larger the N.A, the higher the resolution of the objective (and better

quality of the image) is.

Object to Primary Image Distance

The distance between the object plane and the primary image plane. The conjugate distance is fixed.

Mechanical Tube Length

The distance between the objective shoulder and the ocular shoulder

Infinity

Infinity is an optic lens system that allows the entire field of view to be in focus at the same time, allowing for

higher quality images to be displayed through the microscope. The focal distance is set to infinity between the objective

and the eyepiece prism, also allowing filters to be inserted into the system more easily due to the parallel path of light,

rather than focusing on a specific point.

Technical Parameters