Olympus FLUOVIEW FV300 Overzicht - Pagina 15

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Specifications

Item

Laser light

Visible light laser source

source

Laser combiner

Scanning unit

Scanning method

Field number

Pinhole

Image memory and

scanning speed

Image channel

Selection of filters according to staining

Scanning modes

Image depth resolution

Zoom

Z-drive

Microscopes

Upright

Inverted (special laser safe frame)

External trans-

Transmitted light illumination unit

mitted light unit

Transmitted light detector

Fluorescence illumination unit

PC with system control boards

Fluoview

Image acquisition

application

software

Hardware control

Image display

Image processing

Image analysis

3D visualization

Others

Power consumption

Objectives for BX and IX

(using U-UCD8, IX-LWUCDA and U-DICTS)

Description

W.D

UPLSAPO 4X

0.16

UPLSAPO 10X

0.40

3.1

UPLAPO 10XO3

0.40

0.24

UPLAPO 10XW3

0.40

0.43

UPLSAPO 20X

0.75

0.6

UPLAPO 20XO3

0.80

0.19

UPLSAPO 40X

0.90

0.2

UPLFLN 40XO

1.30

0.2

PLAPON 60XO

1.42

0.15

UPLSAPO 60XO

1.35

0.15

UPLSAPO 60XW

1.20

0.28

UPLSAPO 100XO

1.40

0.12

Specifications

Select from the following laser, to mounted on laser combiner

Multi-line Ar laser (458nm, 488nm, 515nm, Total 40mW), Ar laser (488nm,10mW), Kr laser (568nm, 10mW),

HeNe (G) laser (543nm,1mW), HeNe (R) laser (633nm,10mW), LD405 (405nm, 25mW), LD440 (440nm, 5.3mW)

Each laser light path is equipped with a continuously variable neutral density filter or AOTF

All laser lines are combined to apsis along the same fiber optic

Galvanometer mirror scanners (both X and Y)

20 (10 with use of LD405 laser)

5-position pinhole turret

Standard scanning mode: 256 x 256 (0.45s) - 2048 x 2048 (10.835s)

Bi-directional high-speed scanning mode: 512 x 512 (0.25s) (Simultaneous scanning of up to 2 channels)

Selectable from 2-channel (fluorescence) or 2-channel (fluorescence) + 1-channel (transmitted light)

3-channel (fluorescence) using virtual channel

Manual selection

XY, XYZ, XYT, XYZT, XZ, XT, XZT, point, Line-t, free line-t, line-z, free line-z, Clip, ZoomIn

12-bit (=4096 grey levels)

1X-10X

Step motor/Minimum step 10nm (BX61, BX61WI and IX81 combination), 25nm (other microscope combination)

BX51, BX61, BX51WI, BX61WI

IX81FVSF, IX71FVSF (side port)

External halogen light source connected to microscope via fiber cable

External detector unit with built-in photomultiplier Connected to microscope frame via fiber cable

Connect to external mercury light source and microscope via fiber cable

Standard equipment of FV300-BX51, FV-300-BX61, FV300-BX51WI, FV300-BX61WI

PC-AT compatible machine/OS: Windows XP (English version)/ 1GB memory (can be expanded to a maximum of 4GB)

CPU: Pentium 4, over 2.8GHz, Special I/F board/image capture: PCI bus

Graphic board: G450_Dual 32MB

Hard disk: 80GB 7200rpm_ID (ATA100) with DVD-ROM

Monitor: Two 19" LCD monitors are recommended, each able to display 1280x1024 images in full color (16.77 million colors)

LAN: On board

Scanning condition setting: image size, scanning speed, zoom, panning etc.

Real-time image calculation: Kalman filtering, peak integration,

Laser, scanning unit, microscope

Each image display: Single-channel side-by- side, merge, cropping, tiling, series (Z/T) pass and continuous

LUT: Individual color setting, pseudo-color, Overlay: Lines, text, scale bar, etc

Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement etc.

Calculations: Inter-image, mathematical and logical,

DIC back ground leveling

Overview of fluorescence intensity within an area, histogram, perimeter measurement for user-assigned area,

time-lapse measurement , etc.

3D animation, left / right stereo pairs, red / green stereoscopic images and cross section

Graphic-based help, PAPP (Programmable Acquisition Protocol Processor), time course software (optional),

trigger IN/OUT function (optional), Multi point software (optional)

Microscope (115V 6A/230V 3A), scanning unit+PSU (115V 3.5A/230V 2A), computer & monitor (115V 4.5A/230V 10A),

Ar laser (115V 10A/230V 5A), Mult-linei Ar laser (115V 10A/230V 5A), Kr laser (230V 20A),

HeNe laser each (115V 0.4A/230V 0.2A), LD laser (405nm, 440nm: 100V 0.9A/230V 0.5A)

Condenser for BX Condenser for IX

Cover glass

Immersion

Correction ring

thickness

—

0.17

Oil

0.17

Water

0.17

—

Oil

0.11-0.23

0.17

Oil

0.17

Oil

0.17

Oil

0.15-0.2

Water

0.17

Oil

U-DICTS

U-UCD8A

IX-LWUCDA

position

optical element optical element

U-DIC10

IX2-DIC10

normal

U-DIC10

IX2-DIC10

normal

U-DIC10

IX2-DIC10

normal

U-DIC20

IX2-DIC20

normal

U-DIC20

IX2-DIC20

normal

U-DIC40

IX2-DIC40

normal

U-DIC40

IX2-DIC40

normal

U-DIC60

IX2-DIC60

BFP1

U-DIC60

X2-DIC60

normal

U-DIC60

X2-DIC60

normal

U-DIC100

X2-DIC100

normal

Objectives for fixed stage upright

microscopes (using WI-UCD, WI-DICTHRA)

Objective

N.A.

W.D.

DIC

prism

MPL5X

0.10

19.60

—

UMPLFL10XW

0.30

3.30

U-LDPW10H

UMPLFL20XW

0.50

3.30

U-LDPW20H

LUMPLFL40XW

0.80

3.30

U-LDPW40H

LUMPLFL60XW

0.90

2.00

U-LDPW60H

LUMPLFL40XW/IR

0.80

3.30

U-LDPW40H

LUMPLFL60XW/IR

0.90

2.00

U-LDPW60H

LUMPLFL100XW

1.00

1.50

U-LDPW60H

XLUMPLFL20XW

0.95*

2.00

U-LDPXLU20

Note: These conditions are not met in confocal microscopy

Revolving

nosepiece

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SSNP,

WI-SRE2

WI-SNPXLU