Olympus U-UCD8A Manual de instruções - Página 21

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1. Select the BF brightfield light path (with no optical element engaged).

2. Engage the U-FW32PO polarizer on the U-FWT filter wheel in the light path.

3. Mount the objective to be used in the revolving nosepiece, and rotate the nosepiece to swing in the objective.

4. While looking into the eyepiece, loosen the clamping screw of the U-FWT filter wheel and rotate the U-FWT to

the position where the field of view is perfectly dark. This is the position where the polarizer should be used.

5. Tighten the clamping screw of the U-FWT at the position where the field of view is perfectly dark.

6. Place the specimen on the stage and move the stage up or down to bring the specimen into focus.

7. Adjust the field iris diaphragm opening until it circumscribes the field of view.

8. Stopping down the aperture iris diaphragm may further increase the contrast of the image.

Fig. 4

General Precautions in Operation

1. If reflected light fluorescence microscopy in the U-excitation

mode is performed using a 10X or 20X objective with the

condenser engaged, flare may become prominent depend-

ing on the specimen condition.

If this occurs and if you do not need transmitted light DIC,

either lower the condenser or use the light shield sheet @

provided with the reflected light fluorescence system. (Fig. 4)

2. When the top lens is swung out, the field of view become

obscured if the aperture iris diaphragm is stopped down (when

using a 2X or 4X objective).

3. Due to the large numerical aperture (N.A.), the U-DIC60, U-

DPO60S, U-DIC100 or U-DP100 DIC prism for 60X and 100X

objectives can also be used for brightfield observation.

U-UCD8A