Amersham Pharmacia Biotech INV-907 Anleitung Handbuch - Seite 4
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Seite druckenComplete filling
In this method a surplus of sample is used to
ensure that the sample loop is filled
completely, allowing reproducible sample
volumes. In preparative applications
the sample volume should be at least 2 times
the volume of the sample loop. For analytical
reproducibility a sample volume 5 times
the volume of the sample loop should be used.
With complete filling the sample volume can
only be changed by changing the loop size.
Complete filling is achieved as follows:
1 Set valve to position LOAD.
2 Load the syringe with sample (2-5 times
the loop volume).
3 Gently load the syringe contents into
the loop.
4 Leave the syringe in position. The sample
will be injected onto the column when
the valve is switched to INJECT in
the method.
Note: If the syringe is taken out before
the sample is injected onto the column, self
drainage will occur and the loop will be
emptied.
Emptying the sample loop
When emptying the sample loop a buffer
volume of approx. 5 times the sample loop
volume should be used to flush the loop and
ensure that all sample is injected onto
the column.
Storage
Overnight: The valve can be left filled with a
buffer.
Weekend or Long time storage: Flush the valve
with water and then fill it with 20% Ethanol.
4
4 Maintenance
CAUTION! Only spare parts approved or
supplied by Amersham Pharmacia Biotech
may be used for maintaining and servicing
the valve.
Period
Action
Every 12 months
Change channel plate and
or when required
distribution plate
Cleaning-in-place
Pump a cleaning or sanitizing agent through
the valve. The standard recommendation is to
pump 1 M NaOH for 30 minutes and then
wash out with buffer.
WARNING! NaOH is injurious to
health. Avoid spillage.
Changing channel plate and
distribution plate
A replacement kit, Valve Kit INV–907, is
available, see ordering information in
Reference Information.
1 Ensure that the valve is in position 1 and
then disconnect it from the pump.
2 Remove the 4 screws on the front using
the supplied 3 mm Allen key. Loosen each
one equally in turn so the distribution
plate comes off parallel to the valve body.
Channel
plate
Distribution
plate
3
IN V -9 0 7
Valve INV-907
Instructions 56-8101-01 Edition AC