BD FACSCanto II Kurzreferenz-Handbuch - Seite 2
Blättern Sie online oder laden Sie pdf Kurzreferenz-Handbuch für Medizinische Ausrüstung BD FACSCanto II herunter. BD FACSCanto II 7 Seiten. Flow cytometer
Seite druckenDaily Setup: BD FACSCanto Clinical Software
Set up the cytometer with BD FACSCanto clinical software, even if you will
optimize and run samples using BD FACSDiva software.
1
Prepare BD FACS™ 7-color setup beads.
2
Run the cytometer setup procedure for the bead lot you prepared.
Follow the Cytometer Setup Wizard to complete setup. When
prompted, install the tube containing the setup beads, or the rack
containing the beads tube and optimization samples.
3
If you are running samples manually, unload the tube when prompted.
•
Hold the tube while moving the aspirator arm to the left.
•
Remove the tube from the SIT.
•
Release the aspirator arm and wait for cleaning to finish.
4
(Optional) When setup is complete, view and print the Setup Report.
5
Click
6
Review the Levey-Jennings report for trends in the setup data.
Optimizing Instrument Settings: BD FACSCanto Clinical Software
Before you record data for a sample, optimize the detector voltages,
spectral overlap, and threshold settings for the sample type and
fluorochrome to be run.
1
Follow the prompts on the screen to proceed with optimization.
•
Click Yes to save results.
•
Choose the panel type and the X and Y parameters, and click
.
•
If you are using the Loader for optimization, click
•
Click
•
If you are running tubes manually, install the first optimization tube
when prompted, and click
•
Click
to optimize the settings, or click
at the Cytometer Setup Optimization screen.
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2
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