Apogee 21054010 Gebrauchsanweisung - Seite 4

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1.0

BEFORE YOU BEGIN

1.1

IMPORTANT INFORMATION

The Horizon® 11.14 and 20.25 Blot Transfer Apparatus are designed for capillary transfer of nucleic
acids from agarose gels to membranes in Southern and Northern blots. Used in conjunction with the
appropriate blotting materials and membranes, the included trays and supports offer a simple and
efficient method for performing high quality blot transfers.
Blot transfer apparatus 11.14 is compatible with 11 x 14 cm horizontal gel electrophoresis units
including the Horizon 11.14 and the Model H5 apparatus.
Blot transfer apparatus 20.25 is compatible with 20 x 25 cm horizontal gel electrophoresis units
including the Horizon 20.25 and the Model H4 apparatus.
This manual provides instructions on the use of both blot transfer apparatus noted above.
For operating procedures for the Horizon 11.14, 20.25, H4, and H5 Apparatus, as well as general
guidelines, practical applications, and suggestions for agarose gel electrophoresis, please refer to the
instruction manuals. These systems have not been qualified for use in any human or animal diagnostic
or therapeutic application. Use for other than the intended use may be a violation of applicable law.
Please carefully follow the manual's instructions. Do not alter equipment or operate with broken
components. Failure to adhere to these directions could result in personal and/or laboratory hazards
as well as invalidate the equipment warranty.

1.2 COMPONENTS

The 11.14 and 20.25 blot transfer apparatus are designed for durability and ease of use. Each complete
apparatus includes the following items:
One molded buffer tray
One machined and bent acrylic tray insert
One machined and bent acrylic gel restrainer
One instruction manual
Apogee Electrophoresis | 101 Kane Street | Baltimore, MD 21224 USA | apogeephoresis.com
Figure 1: Components
Gel Restrainer
Tray Insert
Buffer Tray