Olympus CHA Gebrauchsanweisung - Seite 11

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Olympus CHA Gebrauchsanweisung
F. Aperture Iris Diaphragm
An aperture iris diaphragm is provided on the condenser,
the opening of which can be adjusted to match with the
numerical aperture of the objective in use, in order to
70-80%
achieve optimum objective performance, as depth of
focus, image contrast and resolution.
However, since microscopic specimens generally are low
in contrast, their image lacks contrast jf the objective is
used with its full numerical aperture. Therefore, it is
often preferable to stop down the aperture diaphragm
slightly more than indicated by the objective N.A. An
aperture setting at 70% to 80% of the objective N.A. is recommended. If necessary, remove
the eyepiece and, looking at the exit pupil of the objective, adjust the opening of the
diaphragm.
G. Immersion Objectives
To utilize the full numerical aperture of an immersion objective (with engraving "HI" for
homogeneous immersion). the objective, specimen and condenser are immersed in an immer-
sion oil.
*
Care should be taken to prevent oil bubbles from forming in the oil film between condenser,
specimen sl ide or objective.
After use, carefully wipe off the immersion oil deposited on the lens surfaces
with gauze moistened with xylene.
Never leave oil on lens surfaces after use as oil remnants will seriously impair the
performance of the lens systems. It is recommended to use Olympus immersion
oil for immersion objectives.
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