Olympus CK2-BIC Anleitung Handbuch - Seite 10

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Phase Contrast Observation

Legend comes atop.

Legend comes atop,

Fig 15

@-@

Fig 18

Pre aration of the philSe slider

Put the li!tlt annuli for 4X

ill

and 40X

objectives inlO the phase

slider. The annulus@ for lOX is also use<! for 20X objectives. The

annuli for 4X and lOX {lOX

objectives are centered by means of

the centering lever@, (Fig.

15)

Mount the light annulus

for 40X objective into the center hole

of the phase slider (as the ARROW indicates).

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Insert the phase slider

into the slot in the condenser in such a

manner that its engraved surface with figures faces upward. and

the notched side toward the lamp housing pillar. Rotate the aper-

ture iris diaphragm lever

all the way to the left, and insen the

green filter provided intO the filter mount (j). (Fig. 16)

Cantratl

0 tha ;

1) Place a specimen on the

st~

and focus on it.

Remove one of the eyepieces from the observation tube and insert

the centering telescope CT·5

provided into the observation

tube. {Fig. 17)

Ascertain that objective magnification and annular ring are matched.

Rotate the top lens assembly of the centering telescope to bring

the bright ring (light annulus) and the dark ring (phase annulus)

into focus.

Operate the centering lever of the light annulus until both annuli

are concentric and superimposed. (Fig. 181

If a specimen slide or the bottom surface of I cufture ve$Sill is

not flat. either annulus is likely to slip off the center while

centering the other. In such a caSIl. repeat centering adjustment

again. This trouble, however, rarefy occurs with hi!tler magnifica·

tions, so that the 40X philSe annulus needs no centering adjust·

ment.

21 After centering adjustment with the lOX objective, check the

centration with the 20X objective.

3} After centration is complete, remove the CT.s and insert the eye·

piece back into the observation tube.

Now it is ready for phase contrast observation.