Olympus CK40 Anleitung Handbuch - Seite 27

Blättern Sie online oder laden Sie pdf Anleitung Handbuch für Mikroskop Olympus CK40 herunter. Olympus CK40 39 Seiten. Culture microscope

CK40-SL

CK40-RPSL

Fig. 35

Fig. 36

Centering the Light Annulus or

Relief Slit

# The CK40-SLP does not need to be centered.

1. Place a specimen on the stage and bring it into focus.

2. Replace the objective in the sleeve which does not have a diopter ad-

justment ring with the CT-5 centering telescope.

3. Make sure the magnification of the objective in the light path matches

that of the light annulus on the phase slider or that of the relief slit on the

RP slider.

4. While looking into the centering telescope, turn the knurled dial to focus

on the phase annulus ² of the objective corresponding to the light an-

nulus or relief slit @. (Fig. 35)

5. Insert the centering levers | into the two centering tapped holes ³

on the phase slider or RP slider. Tighten and loosen the centering

levers until the light annulus or relief slit @ is superimposed on the

phase annulus ². (Figs. 35 & 36)

6. Repeat these steps to adjust the other objectives. Keep in mind, however,

that 10X and 20X objectives share the same light annulus with the CK40-

SL and CK40-RPSL. Put an uncentered objective into the light path to

make absolutely sure the light annulus or relief slit @ is not deviating

from the phase annulus ². If there is any deviation, perform the centering

procedure again.

# Optimum performance cannot be achieved if the light annulus or

relief slit is not properly centered,

# Ghost images of the light annulus or relief slit may sometimes emerge.

If this happens, superimpose the brightest image with the phase

annulus.

# When a thick specimen is moved or replaced, the light annulus or

relief slit and the phase annulus may deviate. This can reduce im-

age contrast. If this happens, repeat Steps 1 – 5 for readjustment.

# The centering procedure may have to be repeated in order to get

the best possible contrast if a specimen slide or the bottom surface

of a culture vessel is not flat. Center the light annulus or relief slit

using objectives in the order of lower to higher magnifications.

CK30/CK40

(Figs. 35 & 36)