Olympus Fluoview FV1000 Übersicht - Seite 20

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Olympus Fluoview FV1000 Übersicht
Multi-Dimensional Time-Lapse
P2
The FV1000 can be used for ideal multi-dimensional time-lapse imaging during confocal observation,
P3
P1
using multi-area time-lapse software to control the motorized XY stage and focus compensation.
P4
P5
Significantly Improved Long Time-Lapse Throughput
Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system
efficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single experiment to
increase the efficiency of experiments. Microplates can be used to run parallel experiments, which significantly improves throughput for
experiments that require long-term observation.
Focal Drift Compensation for Long Time-Lapse Imaging
The IX81-ZDC Zero Drift Compensation system corrects loss of focus caused by temperature changes around the microscope and other
factors during long time-lapse observation. The thermal drift compensation eliminates the need to take images at several Z planes,
minimizing live cell exposure to irradiation.
Objective
focal
plane
Set target
observation
plane
as offset.
Maintain Cell Activity Over A Long Period
Proprietary CO
incubator control keeps the environment inside the tissue culture dish completely stable. The environment is precisely
2
maintained at 37°C with 90% humidity and 5% CO
0 s
1000 s
Human lymphoblast cells TK6
Courtesy of: Masamitsu Honma, Dir.
Biological Safety Research Center Div. of Genetics and Mutagenesis I, National Institute of Health Sciences
Supports repeated image
acquisition from multiple areas in
a single microplate well.
Multi-Point Time-Lapse Software
Over time, the
Laser detects
objective focal
the glass
plane drifts from
surface before
the observation
imaging.
plane.
concentration.
2
2000 s
3000 s
Point 1
Focal Plane 4
Focal Plane 3
Focal Plane 2
Focal Plane 1
IR Laser for focal
plane detection
Immediately
returns to initial
offset plane,
for focal drift
compensation.
4000 s
5000 s
19
Point 2
Point 3
Point 5
Point 6
Baseline focal plane
ZDC
Scanning
unit
6000 s
7000 s
Point 4
Offset