Olympus FLUOVIEW FV300 Übersicht - Seite 9
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Multi-point time lapse system
High-magnification multi-point time lapse observation of living cells.
Introduction of DsRed2 expression vector into brain of 14th day
mouse embryo through womb electroporation. Taking a specimen
slice from the 15th day embryo and observing the living nerve stem
cell and nerve cells subsequently born from it.
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Using multi-point software*, it is possible to acquire an XYt, an XYZ or and XYZt image series
at multiple positions through automated software control of the motorized XY stage.
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* Multi-point software and motorized XY stage are optional
Image courtesy of:
Dr. Kazunori Nakajima
Dr. Hidenori Tabata
School of Medicine, Keio University
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Wide-ranging specimen observations
for improved experiment throughput
Use of a motorized XY stage allows the
analysis of time lapse changes in many
points scattered over a wide area. The
system is therefore effective for work with
thick specimens, such as observing
changes in the states and movements of
stem cells using a brain slice, or analyzing
expression mechanisms at the individual
level in an embryo. In wide-ranging tasks
such as analyzing cell functions using GFP,
the system provides many different kinds of
data at the same time, enabling a higher
overall level of experiment efficiency even in
long-lasting observations. In addition, using
separate chambers at the same time makes
it possible to perform different experiments
at the same time. These are just some of
the ways in which this system dramatically
improves the throughput of experiments
requiring long-duration observations.
Features
1. Measure up to 254 points
A variety of scan conditions can be set for each point,
such as XYZ coordinates, the Z-axis acquisition range
and the detector sensitivity.
2. Up to 5 X 5 adjacent fields of view registered
automatically
Since adjacent fields of view are registered
automatically, it is possible to broaden any given field
while maintaining a high magnification level.
3. High-precision XY stage scanning
A wide area can be observed with highly precise position
reproduction. Errors from repetition are not
accumulated. (High-precision XY stage complies
exclusively with the "PROSCAN" model from PRIOR
Scientific)
4. Flexible scanning conditions
The system combines scan flexibility with time lapse
imaging for monitoring changes in the specimen over
time.