Olympus FV3000 Betriebshandbuch - Seite 15

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Multi Area Time Lapse

（with the Z drift compensation）

⑦

⑩

*Before starting the following procedure,

make adjustments for XY imaging.

ZDC Setting (See page 10 for details. )

① In [▼Z Limit Setting], press the

Near limit to all objective lenses.

② In [▼Z Section] in [Series] Tool Window,

press the

⑤ Set [ZDC DM] in [▼ZDC Control] in

[Microscope] Tool Window to "In" and

place the dichroic mirror of ZDC in the

light path.

⑥ Specify the search zone of the coverslip

position in [Upper Limit] / [Lower Limit]

in [Search Zone] using the Origin

coordinate of Z as a base point.

⑦ Set [Z Drift Compensation in series scan]

in [ZDC Control] in [Microscope] Tool

Window to "ON".

⑧ Adjust focus and Press the

button of [Coverslip position]. The

coverslip top surface position is acquired.

When the Z drift compensation is

successful, the buzzer sounds beeps

once only. If the Z drift compensation is

not successful, the buzzer sound beeps

three times.

⑨ Press the

button to register areas.

⑩ set [Cycle interval] and [Cycle].

Starting Multi Area Time Lapse

⑪ Select [MATL] tab in [Acquire] Tool

Window.

⑫ Press the

button to display the dialog

box, and select the folder to save the

images.

⑬ Press

button to start acquiring the

image.

◆motorized stage◆

◆ZDC◆

button to set the

to set current position 0.

⑪

⑬

⑫