Olympus FV3000 Sicherheitshandbuch - Seite 13

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• Export Images:

➢ Right click on the image and

then export. An Export

windows will pop up.

➢ Select your directory where

you would like to save your

TIFF images into.

➢ Save as type: TIFF (*.tif, *.tiff)

➢ Under Output format, select

RGB Color with merge and

24bit Full color.

➢ Click Save.

4.10 Switching OFF Protocol

Check through TLL microscopy resource booking if there is a user immediately after you. If

there is a user immediately after you, leave the system on and log off the PC. Otherwise

switch off the system.

(a) Save your images through TLL common drive folder. Images saved in computer D/E

drives will be deleted within a month without prior warning.

(b) Lower down the objective lens completely. (Z value= 0)

position.

(d) Carefully clean the objective lens with Whatman lens tissue using 100% ethanol.

(e) Switch lens back to 10x/0.3 objective lens.

(f) Exit the FV31S-SW software and shut down PC (2).

(g) Press "OFF" on the touch screen panel and wait for 5 secs for the light to disappear.

Then, switch OFF the power button at the back of touch screen panel (6).

(h) Switch off the Stage controller box (3), CBH controller (4).

(i) Switch off the HBO/Mercury lamp if used (5). (Record down the lifespan hours in the

logbook.)

(j) Switching OFF lasers,

•

To turn off 405/488/561/640nm, turn off the individual laser lines and turn the

key to "standby", (8). Switch off the main combiner (7).

•

To turn off 445/514nm, turn off the individual laser lines and turn the key to

"standby" (10). Switch off the sub combiner (9).

(k) Turn off the incubator (12) if it was on.

(l) Finally, Switch off the master switch on the wall (1).

(m) Fill in the logbook with the objective lens used.

Last updated on Mar 2022