Olympus IX70 Betriebshandbuch - Seite 27

Blättern Sie online oder laden Sie pdf Betriebshandbuch für Mikroskop Olympus IX70 herunter. Olympus IX70 32 Seiten. Inverted fluorescent microscope
Auch für Olympus IX70: Betriebsanleitung (5 seiten)

Seite drucken

NOTICE: MF can sometimes 'forget' the camera is 12-bit (senile code) and the images will come

up as 8-bit (see scale on side of image or histogram); it's a bug. This happens if you don't load a protocol

before hitting NEW, or actually create a new protocol. To correct it, you need to close the experiment, use

the File/select video channel/camera and re-select Retiga monochrome-12 bit (even though it looks

selected). You will be back to normal. So, the first task in creating a new protocol is to go to FILE\select

camera and re-pick 12-bit mode.

You should set the sampling time you desire in the Expt Control Panel button 'Set Timelapse'.

You can enter the total number of points you desire or the duration. Entering "0" for # points makes the

collection run until stopped; you still want to tell it how often to sample, though. You can check the

parameters by using the Acquire One sidemenu button (it grabs ONE SET). The other buttons below it

grab only the associated wavelength (1-5).

Be sure to check the boxes for logging ROI data (we're getting there next) and images otherwise it

runs and you save nothing. The first time you check a box, MF will ask you for a filename and location.

Data can be sent to MS Excel and/of a text file. The Save images enables storage of anything (and at

interval) you set in the 'Cfg Expt' menu. The little lights to the right will be colored for data channels that

will be saved. MF hasn't evolved from DOS days, so naming schemes still have to be limited to 7

characters (it appends a 'd' to the 'inf' name and a 1-5 to describe the wavelength to images, and the file

descriptor is sequence #)(the inf file contains timing info, event marks, etc; don't get rid of it).

locations for reuse, else the location of the upper left point is stored in data logs (but no shape/size info).

Set thresholds on one or more channels to exclude background from ratio calculations. Only data

above (each) threshold are used in calculations. Only data that are below the top limit are used as well (so

saturated pixels drop out giving wrong impression that average ratio is dropping; a good reason to save

images and check for this). Press the 3bar icon, pick autothreshold for light objects and adjust blue

triangles, if needed, to delimit "signal".

Zero the elapsed time clock (Expt Cntl Panel), then hit Acquire or the F2 key (F4 pauses).

PDF Created with deskPDF PDF Writer - Trial :: http://www.docudesk.com

CLEAR all

ROI

Next define ROIs and any

calibrations and thresholds. Hit the

Regions button on the sidemenu. The next

dialog lets you pick the channel to define

ROIs on and to collect an image if you

haven't done that yet. Pick a drawing tool

from the icons at the top (I like circle),

start at upper left of where you want to

draw and you can stretch the size by

holding left mouse button and dragging

down and right. You can right-click over

ROI icons to set default size (make this

smaller than you normally want, maybe 5-

10 pixels, since it's easy to stretch it

during creation). The closed, squiggly

icon is the freehand draw; you can draw

while holding the left mouse button down

(continuously) or tack down a few points

to have linear spans connecting the 'dots'.

You need to double click to stop the ROI.

ROI can overlap. You can save the ROI