Olympus IX81 Broschüre & Specs - Seite 12

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Manipulator

Manipulating cells.

Micromanipulation system/ON3

Olympus' original micromanipulator offers high stability and

excellent stability because of its compact body. Motorized coarse

and oil hydraulic fine three axes operation are designed in its

compact and rigid body with hanging down ergonomic joystick

control. ON3 micromanipulator is securely fixed through the screw

holes on IX2 microscope frame.

ON3-99D

ON3-99D with return mechanism (UT-R)

Manual combination

Manual combination with return mechanism

(ONM-2D+ONO-301D+UT-D)

(ONM-2D+ONO-301D+UT-D+UT-R)

Laser Scanning Confocal

Simultaneous laser light stimulation and imaging.

Confocal laser scanning microscope/

FLUOVIEW FV1000 system

The Fluoview/FV1000 is a next-generation imaging system

designed for high-resolution, confocal observation of both fixed and

live cells. The FV1000 offers advances in confocal system

performance while providing the speed and sensitivity required for

live cell imaging with minimal risk of damage to living specimens.

In addition, the FV1000 offers a revolutionary synchronized laser

scanning system called the SIM Scanner.

While one laser stimulates, the second laser

simultaneously provides high resolution

imaging. This coordination of laser

stimulation and imaging makes the FV1000

an ideal choice for FRAP,

FLIP and photoactivation.

* FV1000 is a class 3B laser product.

Human embryo

TIRFM

Ultra-sensitive fluorescence microscopy.

TIRFM

(Total Internal Reflection Fluorescence

Microscopy)

Since 1997, Olympus has been a market leader in objective based

Total Internal Reflection microscope that allows an evanescent

wave illumination approximately 200 nm into the specimen beyond

the coverglass interface. Olympus extends that leadership role by

offering four objectives for TIRFM including the world's highest N.A.

objective, the 100X N.A. 1.65 objective.

The incredibly thin optical section created be TIRFM allows an

extremely high signal to noise image to be collected. Popular

applications include vesicle tracking, cellular adhesions and single

molecule events.

• Olympus' original high N.A. objectives make it easy to produce an

evanescent wave field. So little light is leaked that a high-contrast

image can be obtained against a dark background.

N.A. 1.65, 100X objective (APO100XOHR)

(Use special cover glass and immersion oil)

N.A. 1.49, 60X objective (APON60XOTIRF)

(Use normal cover glass and immersion oil)

N.A. 1.49, 100X objective (UAPON100XOTIRF)

(Use normal cover glass and immersion oil)

N.A. 1.45 150X objective (UAPON150XOTIRF)

(Use normal cover glass and immersion oil)

• Once the initial alignment of the laser

optical path is set, it is just simple operation

switching between TIRF and widefield

illumination.

* TIRFM is a class 3B laser product.

Application System

Images of Kaede-expressed cells demonstrating

the photoactivation acquired every 300 msec and

observed via 405 blue diode laser illumination with

twin scanners.

Total Internal Reflection Fluorescence

Widefield fluorescence observation with

observation with evanescent wave excitation

mercury arc lamp excitation

Micrometer