Olympus U-25ND25 Anleitung Handbuch - Seite 11

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USING THE CONTROLS

1. Verify that the power supply voltage and frequency match the requirements inscribed on the rating plate.

2. Make sure that the power cord and connecting cables are plugged in securely.

3. If you perform only transmitted light phase contrast or transmitted light DIC observation, leave one fluorescence mirror unit

position on the turret empty. This allows for transmission of original color reproduction.

4. Enlarge the field iris diaphragm so it just circumscribes the field of view. If decentered, center it using the Allen screwdriver.

5. Always use immersion oil for immersion objectives.

6. If you use an objective with correction collar such as the UPlanApo40X or PlanApo40X, you can correct variations in cover

glass thickness by adjusting the correction collar.

Collection procedure

7. Engage the shutter if you interrupt observation for a short time.

(Turning the mercury burner ON and OFF repeatedly will significantly shorten the life span of the burner.)

8. Color fading of specimens:

This system features high excitation light intensity to ensure bright observation of dark fluorescence specimens.

In consequence, after long period of observations using high-power objectives, the colors of specimens will fade quicker

than usual, causing the view (contrast of fluorescent images) to deteriorate.

In such a case, slightly reduce the excitation light intensity to slow color fading down and improve the fluorescence

image.

To reduce the excitation light intensity, use ND filters or aperture iris diaphragm as far as the observation is not affected or

use the shutter to limit the exposure of specimen to more than necessary light.

Commercially-marketed color fading protection agent (DABCO, etc.) can also delay fading of specimen colors. The use of

fading protection agent is recommended especially when you perform high-magnification observations frequently.

# Remember that fading protection agents cannot be used with certain kinds of specimens.

Selecting the Fluorescence Mirror Units

Select the fluorescence mirror unit which matches the fluorochrome in use.

# Never mount or use the U-MBF3 brightfield mirror unit with a fluorescence mirror unit. The U-MBF3 is excessively

bright and injury to the eyes could occur. If this type of mirror unit is to be used together with a fluorescence mirror

unit, use the U-MBFL3 mirror unit equipped with a built-in ND filter or add a 3% ND filter to the U-MBF3.

}Use of fluorescence mirror units according to the excitation wavelength

Olympus has prepared some sets of fluorescence mirror unit combined with appropriate filters which are variable de-

pending on wavelengths.

The wide-band (W) set is normally used. There may be cases, however, where superwide-band (SW) or narrow-band (N)

sets are recommendable.

@Extremely weak fluorescence brightness

(B- and G-excitation only)

²Specimens emitting strong autofluorescence

Turn the correction collar while adjusting the fine adjustment knob to where the image is

focused. Cover glass thickness for which correction is possible is from 0.11 to 0.23 mm.

Use the superwide-band (SW).

}With the SWB2, strong autofluorescence may reduce

image contrast.

Use the narrow band (N).

}The fluorescence brightness is somewhat reduced.