Olympus U-UCD8 Anleitung Handbuch - Seite 10

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7. Reduce the field iris diaphragm opening until its image circumscribes the field of view.

8. Adjust the aperture iris diaphragm.

# If the slide glass is thicker than 1.2 to 1.4 mm, the image of the field diaphragm may remain fuzzy.

When performing photomicrography, use a side glass with a thickness between 0.2 and 1.2 mm

whenever possible.

Aperture iris

diaphragm image

Objective pupil

Fig. 1

Field Iris Diaphragm

70-80%

· The field iris diaphragm controls the size of the illuminated

30-20%

area. By stopping down the field iris diaphragm, in accordance

with the objective in use, until its image circumscribes the

field of view, stray light can be reduced, which in turn increases

the definition and contrast of the image.

Aperture Iris Diaphragm

· The aperture iris diaphragm controls the numerical aperture

(N.A.) of the illuminator. In order to achieve the optimum objec-

tive performance, the opening of the aperture iris diaphragm

should be matched with the N.A. of the objective in use. This

will result in better image contrast and resolution as well as

increased depth of focus.

}When using an oil immersion type top lens, read the upper

graduations (marked "TLO") on the aperture iris diaphragm

scale. When using a dry type top lens, read the lower gradua-

tions (marked "TLD") on the aperture iris diaphragm scale.