Nikon N-STORM Betriebshandbuch - Seite 21

Blättern Sie online oder laden Sie pdf Betriebshandbuch für Mikroskop Nikon N-STORM herunter. Nikon N-STORM 48 Seiten. Super resolution microscope
Auch für Nikon N-STORM: Benutzerhandbuch (11 seiten)

Seite drucken

Finely and properly adjust the laser focal point of the

TIRF illuminator.

(1)

Select the checkbox for the 647-nm laser.

(2)

Turn on the Perfect Focus System (PFS) in [Ti Pad]

and set the focus.

(3)

Finely adjust the laser focal point of the TIRF

illuminator so that the observation target is visible.

Check activation.

(1)

Make sure that the checkbox for the reporter laser

(the figure on the right shows an example when a

647-nm laser is used) is selected, and then set the

power to 100% by adjusting the power slider.

(2)

Make sure that the checkbox for the activation laser

(the figure on the right shows an example when a

405-nm laser is used) is deselected, and then set

the value to about 0.3% by adjusting the power

slider.

(3)

Select the checkbox for the activation laser so that it

emits laser light for about a second, and then

immediately deselect it.

Check that the live image becomes bright once and

then returns to the previous state as a result of

activation causing the fluorescent probe to

fluoresce.

If activation cannot be checked

If emission of the activation laser does not make the live

image bright, this can be improved by replacing the

buffer solution of the specimen with a fresh solution.

Chapter 2

STORM Microscopy Operation

3-(1)

4-(3)

3-(2)

3-(3)

4-(2)

4-(1)