Nikon N-STORM Betriebshandbuch - Seite 42

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Icon

[Start STORM Analysis]

[Apply Drift Correction]

[Display 3D STORM

Image]

[Display Options]

[Filter Settings]

[Create New Elements

Document from Current

View]

[Help]

[1 to 1 Scaling]

[Zoom In]

[Zoom Out]

Left tool bar

Icon

[Show conventional low

resolution image]

[Auto Scale conventional

low resolution image]

[Switch STORM high

resolution image display

mode]

[Mark molecules identified

in current frame]

[Adjust Gaussian

rendering parameters]

[Continuous automatic

Gaussian rendering

parameters adjusting

mode]

[Single shot automatic

Gaussian rendering

parameters adjustment]

[Drift corrected

coordinates display]

Chapter 3

The Screens of the N-STORM Software

Name

Starts STORM analysis and drift correction.

Only performs drift correction.

Displays a 3D image (3D-STORM only).

Determines how to display an image.

Sets the conditions for displaying only some molecules.

Outputs a new NIS-Elements document from the current view.

(The state of zoom and the display modes of conventional

images and STORM images affect the output image.)

Displays help.

Adjusts the zoom level so that one pixel of the image matches

one pixel of monitor.

Zooms in on the display of the image.

Zooms out on the display of the image.

Name

Selects whether to display a conventional image.

Selects whether to enable or disable auto scale (auto

adjustment) of the intensity for displaying a conventional image.

(If auto scale is enabled while ROI is being used, it is adjusted

according to the ROI range.)

Determines how to display a STORM image ([None] (Not

displayed)/[Cross]/[Gaussian] (Gaussian representation)/[Both])

Marks, with a yellow square, a molecule identified in the current

frame of the conventional image.

Displays a dialog in which settings are configured for the

Gaussian representation. Adjusts the Gaussian representation

according to the intensity and size of the bright point images.

Set this button to on to automatically adjust the Gaussian

representation according to the current display range.

Clicking this button automatically adjusts the Gaussian

representation according to the current display range (the ROI

range if ROI is used).

Applies drift correction to the displayed image (only available for

STORM images on which drift correction is performed).

Description