Etaluma LS460 Startup-Handbuch - Seite 9

Blättern Sie online oder laden Sie pdf Startup-Handbuch für Mikroskop Etaluma LS460 herunter. Etaluma LS460 14 Seiten. Lumascopes with lumaview (for use with ls560, ls460, ls600, ls620, ls720)

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Not checking Manual image autosave will result in opening of the Save As dialog box every time the

camera icon is clicked and the image has been snapped. In this case, the file name must be entered

before clicking Save.

8. On the right side of Instrument Setup, checking Display scale bar results in a scale bar in the lower right

corner of the live image and snapped images. If checked, Select Color, check Display objective power if

desired, and select Scale bar width.

Be sure to click OK to save the settings before exiting Instrument Setup.

If a different Frame Size was chosen in Step 4 above and OK is clicked in Frame Size, a communication

dialog box will open directing you to shut down and restart Lumaview. Click OK and then shut down

Lumaview. Upon restarting Lumaview, the frame size in the lower status bar will be the new size. If a

different frame size is selected and the computer is not restarted, the new frame size may show in

Instrument Setup but it may not have actually changed. Always check the lower left status bar of Live

Image Window to make sure your desired changes have been incorporated.

D. Finding a Live Image

1. Place sample on deck. Manual XY movement of SBS labware (microplate footprint) is facilitated by

Etaluma's Manual XY Stage (optional). For labware that is not SBS, use a labware holder such as the

Multi-Slide Holder for 1-4 microscope slides.

2. Click on the Manual Image icon in the Left Toolbar (top icon) to open its dialog

box (LS460 dialog box contains only the Brightfield controls). If desired, move

dialog boxes to either side of the Live Image Window. If dialog boxes are closed

and then reopened, they will open in the same locations as they were prior to

closing. However, when Lumaview is closed, the dialog box positions will not be

saved.

3. In Manual Image, select the desired channel. For fluorescence, the included Shroud (or other

protection from light) must be placed over the labware. Start with Exposure at maximum and Gain as

low as possible. Increase Illumination gradually to the desired brightness. For dim samples, if

Illumination is at maximum, increasing the Gain can be used to increase signal but it will also increase

the background. Illumination can also be increased by using the external power supply/cord (See

Section I.G.).

For brightfield using ambient light, start with Gain as low as possible and adjust Exposure until a mid-

gray tone is seen in the Live Image Window.

For brightfield using the Phase Contrast Accessory (use open position in Slider, see Section I.F.), check

Brightfield in Manual Image and turn the phase condenser iris (on front of condenser) so only a low

level of light is transmitted. Set Gain to minimum, Illumination (%) to minimum (e.g., 0.4%), and then

adjust Exposure until a mid-gray tone is seen in the Live Image Window.

2017.11.20

LS560/460 Startup Guide