Etaluma LS400 Startup-Handbuch - Seite 8
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H. Capturing time-lapse series
1. It is first necessary to download and install the following two freeware programs.
a. AviSynth video post-production program found on the AviSynth Wiki at
http://avisynth.nl/index.php/Main_Page. Download and install the Official Build. The default save
location for the program will be the Etaluma folder inside the Program Files (x86) folder.
b. MeGUI video conversion program found at http://sourceforge.net/projects/megui/. After the
zipped folder (MeGUI_2525_x86.zip) has downloaded, click to open and Extract All. Depending on
your computer and Windows version, it may be necessary to right click on the .zip file to complete
the extraction. After extracting the files from the zipped folder, you will see the file MeGUI
Application with a green icon in the MeGui_2525_x86 folder. Extraction so that the green icon is
shown is essential for the images to be scripted correctly for video compiling. If desired, make a
shortcut on your desktop.
2. Before initiating a time-lapse study, review Computer Configuration in the Help Section and change
your power and Windows update settings so they do not automatically turn on and interfere with
scheduled image captures. Of particular importance are Sleep Mode and Auto Restart. After time-
lapse is completed, be sure to change the settings back.
3. With cursor over Live Image Window, right click and select Time Lapse Settings to open its dialog box.
4. For brightfield images, check White in the Activate column (be sure FL is not checked). Check Auto Gain
(ignore Gain % and Exposure %). Do not click on Load Present Values.
5. For fluorescent images (not applicable to the LS400), check FL in the Activate column. To set LED %,
Gain % and Exposure values, use desired levels determined in Section II.E above or determine now and
click Load Present Values. Time Lapse can also include brightfield in a darkened space (will require one
of the 3 light sources described in Section II.D.2), in which case the White box would also be checked as
well as its Auto Gain.
6. Choose Saving Destination Folder, Filename Root, and Filename Starting ID number. The Destination
Folder should be unique for each time-lapse because of how the images are compiled into scripts. File
format is selected in the Images dialog box accessed by right clicking over the Live Image Window and
selecting Image Parameters.
7. Set Duration and Interval values and LED Pre-Snap Time (a typical time is 1 second). Images will be
numbered sequentially and collected in the Destination Folder along with an Avisynth (.avs) file
containing a compiled script of the captured images.
8. Be sure to click Save.
9. Start series by clicking on clock icon in top tool bar of Live Image Window. Depending on the Lumaview
version, you may see another dialog box open asking if you wish to start time-lapse. Click yes. To stop
image capture at any time, click on clock icon again. You may be asked if you wish to stop time-lapse
and if so, click yes.
I.
Compiling time-lapse images into videos
1. Click on the MeGUI Application to open the program (may take several minutes).
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Lumascope 500/400 Startup Guide
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