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LSR-II 20L Pump System Instructions (Cytek)

Fluid managers are in use on all our LSR IIs. Using the pump offers the following advantages:

• No need to open and fill a metal tank.

• Stabilizing of sheath flow rates allow use of a 6 uSec window extension.

To use:

1. The pump system turns on automatically with the LSR.

2. Verify that the pump is on. The LSR will not work properly otherwise.

Its switch is located on the blue box labeled "Cytek" under the LSR with the black lever switch.

3. Check the sheath tank. Fill the sheath tank if there is not enough for your run.

4. Check the waste tank. Empty the waste tank if full.

5. Please email [email protected] with any problems or questions

Changing the tanks:

1. The cube is on a scale, so you can check the weight of the cube (are there guidelines for what the

readout should be? Instrument use is ~1L/h or 2.2lbs).

2. If the sheath cube is empty replace it with a new one (it is heavy so lift carefully) New cubes are

located in either the hall or the rear of the room.

Note: The cube closure just screws on – there is no pressurization of the cube. Be careful not to kink the

tubing. Turn the outer ring only so you do not stress the wires for the sensors.

3. Empty waste in sink in hallway when full, using the protective face shield, gloves and lab coat.

4. Alarm sensors are connected to the cube and the waste, indicating when they need attention if you

forget. The LSR won't run samples properly if the pump is turned off.

Diva Software Instructions

1. Log into the Diva software using BDFACSDiva software icon on the desktop

Username is Sunet ID/ password starts with a capital letter

2. Watch for the "Instrument connected" status in bottom of the Cytometer

3. If CST mismatch dialog box appears select use

Setting Up Your Experiment

1. Create an Experiment: Select Experiment®New Experiment®Select the SSFF tab®choose the

newest date available®select experiment Click® OK

2. To Rename -- right-click ®Rename Use descriptive name and date

3. Edit menu®User Preferences®Select desired defaults.

You can deselect save analysis and load data after acquisition to stop instrument from reloading data after

Remember: Add bleach to line marked on carboy.

The pump should NEVER be turned off manually!!

acquisition. This helps if you are recording large data files.

CST settings

window