Beckman Coulter Vi-Cell XR Instrucciones de uso - Página 8
Navegue en línea o descargue pdf Instrucciones de uso para Instrumentos de medida Beckman Coulter Vi-Cell XR. Beckman Coulter Vi-Cell XR 9 páginas. Cell viability analyzer
Imprimir página- 1. Getting Started with the VI-Cell
- 2. System Specs
- 3. The Software
- 4. Running a Sample
- 5. Camera Image
- 6. Autosampler Queue
- 7. Sample Prep
- 8. Analyzing the Sample Run
- 9. Saving Data
- 10. General System Information
- 11. VI-Cellxr Cell Image Analysis Scheme
- 12. Creating and Modifying VI-Cell Cell Types
- 13. To Access the Cell Types
- 14. Sample Prep Options
- 15. Image Analysis Parameters
- 16. Cmcresson
CMCresson
Parameter
Range
Images
1 – 100
Size
3 - 70 µm
Brightness
75 – 90
Sharpness
60 – 200
Spot
Brightness
Spot Area
1 – 20 %
Minimum
0–0.5
Circularity
None, low,
Decluster
med, high
Default
50
Increase value for very low concentration samples
If a particular cell is being problematic, place
10 -40
cursor over cell and size is displayed below
µm
image
Picks out objects from background
85
Increase includes more objects
Decrease includes less objects
Picks out objects from background
100
Decrease to include more objects
Increase to include less objects
Determines cell viability
75 %
Increase value more cells counted as dead
Decrease value more cells counted as live
Ratio of the bright spot to total area
Determines cell viability
5 %
Increase value more cells counted as dead
Decrease value more cells counted as live
Excludes dead cells so they are considered debris
0
0 excludes no dead cells
1 excludes all dead cells
Ability of software to pick individual cells from a
medium
cluster of multiple cells
None counts a cluster as 1 cell
15-March-06
Details