Beckman Coulter CEQ 8000 Manual de preinstalación - Página 14
Navegue en línea o descargue pdf Manual de preinstalación para Instrumentos analíticos Beckman Coulter CEQ 8000. Beckman Coulter CEQ 8000 16 páginas. Genetic analysis system
Imprimir páginaMaterials Required but not Supplied
Materials Required but not Supplied
Refrigerated microfuge
Molecular Biology Grade: sterile dH
(v/v) ethanol/ dH
O
2
Sterile tubes - 0.5 mL microfuge, 0.2 mL thin wall thermal cycling tubes or
plates
Thermal cycler with heated lid
CEQ Sample Loading Solution (SLS)
Microduster III AccTech, P/N: 58019-538 (VWR)
Mini Alpha Swab from Texwipe, P/N: TX754B (VWR)
20 mg/mL Glycogen - Boehringer Mannheim Cat. # 901 393
3M Sodium Acetate pH5.2 - Sigma, Cat. # S7899
100mM Na
-EDTA pH8.0 (500 mM Sigma Cat. # 7889)
2
PCR enzyme and buffer
Labeled primers (available from Research Genetics)
Optional Materials
•
Aluminum Foil Seal and Sample, P/N 538619
•
Thermowell™ Cap Strips - Corning Cat. # 6556 (not supplied by Beckman,
Coulter, Inc.)
Training Plan
Basic training should take place immediately following the installation of the system.
The Beckman Coulter Field Engineer will train two users in basic instrument
operation, use of the software program and routine maintenance. (Advanced training
sessions may be scheduled for an additional fee. Contact your local Beckman Coulter
representative for more information.)
To optimize the training session, the following prerequisites have been established for
users:
•
Experience in the handling of biological samples
•
Working knowledge of the Windows® Operating System
•
Familiarity with DNA sequencing and/or fragment analysis
Beckman Coulter Field Engineers do not provide Windows training.
10
O, 95% (v/v) ethanol/dH
2
CEQ™ 8000 Genetic Analysis System
Sequence
Analysis
O, 70%
2
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Fragment
Analysis
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