CORNING MicroDEN Manual de instrucciones - Página 3

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L'utilisation de cet appareil est soumise aux conditions suivantes: (1) cet

appareil ne doit pas provoquer d'interférences et (2) cet appareil doit tolérer

les interférences externs, y compris celles quie peuvent provoquer un

fonctionnement anormal de cet appareil.

Intended Use

The Corning® MicroDEN® unit is intended to be used in a laboratory

environment by personnel trained in proper laboratory procedures.

Equipment Installation

The MicroDEN unit is designed to be used on a laboratory benchtop, powered

by the supplied external power supply. Up to 4 MicroDEN units may be placed

inside a standard cell culture incubator at 37°C.

When loading and unloading the MicroDEN unit, be sure to follow all laboratory

safety requirements including the use of personal protective equipment and

flow hoods.

Manufactured for Corning by:

Flaskworks

38 Wareham Street, 3rd Floor

Boston, MA 02118

USA

1. Introduction

Corning® MicroDEN® unit is designed to differentiate monocytes into dendritic

cells. Its innovative design allows for easy cell differentiation without the use of

traditional well plates, T-flasks, and bags.

Designed for Efficient Cell Culture

The Corning MicroDEN unit can be installed within several minutes, with minimal

training, and requires minimal user interaction once the process is set up.

Operating Range

Corning MicroDEN can be used to differentiate mammalian monocytes into

dendritic cells with a seeding density range of 200,000 to 800,000 monocytes

per cm

per Corning TC-treated perfusion cell culture flask, with an approximate

dendritic cell yield of 15% to 30% of input monocytes from a healthy human donor.

Overview

1. Corning MicroDEN unit (Cat. No. 431276)

2. Single-use consumable kit includes:

Two Corning TC-treated perfusion cell culture flasks (Cat. No. 431277)

◗ –

in thermoform tray

Two Corning 150 mL easy grip polystyrene storage bottles with dip tubes

◗ –

(Cat. No. 431278)

2. Getting Started

Product Contents include the following items:

1. User Manual

2. Corning MicroDEN unit

3. AC adapter

3. Material Requirements

1. Corning MicroDEN unit (Cat. No. 431276) requires standard electrical outlet

2. Corning consumable set (Cat. No. 431277)

3. Corning storage bottle (Cat. No. 431278)

4. Enriched monocytes (MOs)

5. Differentiation medium containing IL-4 and GM-CSF (185 mL)

25.0 mL for seeding

◗ –

80.0 mL at start-up for perfusion

◗ –

80.0 mL on Day 3 for perfusion

◗ –

6. Cold buffer (4°C) for harvest

PBS [-] Mg

and Ca

◗ –

7. Biosafety cabinet

8. Incubator, 37°C, 5% CO

9. Vortex mixer or shaker (optional)