A-M Systems 3000 Manual de instrucciones - Página 11
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connector is less than ±10 V, otherwise higher and/or lower portions of the signal
may appear cutoff or flattened.
Stimulating
The
switch should be placed in the
MODE
monitored by measuring the voltage (V) across an internal, fixed resistor (R = 100 Ω)
in series with the electrodes. Since I=V/R and R is known, the voltage is a measure of
the stimulus current. The internal resistor is in series with the indifferent lead (
so that excessive voltages do not appear at the Probe Amplifier inputs. Therefore, to
ensure that the current in the indifferent lead is equal to and opposite of that in the
active lead, only isolated stimulus sources should be used. An added benefit of
isolated stimulus sources is that they produce less stimulus artifact than ground
referenced sources. The Positive Conductor of the Stimulus connector (center pin) is
connected through the Probe to the active electrode lead, while the Negative
Conductor (outside ring of the BNC) is connected to the indifferent electrode lead.
When an isolated stimulator is used the
function properly
Mounting Micropipettes
The easiest way to use a micropipette as an electrode is to place the micropipette in
a micropipette holder with a 2mm pin. It will fit firmly into the headstage (+) input
connector.
Mounting the Head stage in a Micromanipulator
The Head stage should be clamped in the manipulator by means of the mounting rod
supplied. The mounting rod can be screwed into the cable end of the head stage for in-
line mounting.
A-M Systems
STIM
PROBE-
131 Business Park Loop, P.O. Box 850 Carlsborg, WA 98324
Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525
E-mail: [email protected] * Website:
position. Stimulation current (I) is
connector must be grounded to
http://www.a-msystems.com
)
PROBE-
8