Olympus 1X71 Manual del usuario - Página 9

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Adjusting the objective correction collar

Correction is possible according to the vessel bottom thickness.

1. When the thickness of the vessel bottom is known, match the scale reading of the

correction collar to the thickness of the vessel in use.

2. If the thickness of the vessel is unknown or diverge from the manufacturer

specifications, the optimum position for the correction collar can be obtained by

judging the image resolution and contrast. When a satisfactory image is not obtain

after focusing:

1. Rotate the correction collar to the left and right, refocus each time and

compare the images.

2 Then rotate the collar in the direction yielding a better image, rotate the

correction collar to the left and right, refocus each time and compare the

images.

3 Repeat this cycle until the position with the optimum image is found.

20x Correction Collar

Correction Collar Scale

0 mm

0.17 mm (glass coverslip #1.5)

0.5 mm

1 mm

(most tissue culture plates)

1.5 mm

2 mm

40x Correction Collar