Olympus BX-FLA Manual de instrucciones - Página 5
Navegue en línea o descargue pdf Manual de instrucciones para Microscopio Olympus BX-FLA. Olympus BX-FLA 46 páginas. Reflected light fluorescence attachment
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The design of reflected light fluorescence
microscopes features dichroic mirrors which direct the excitation light
through the objective, to the area of the specimen, thus providing efficient illumination. (Fig. 1)
Eyepiece
II
I
I
I
>
Objective
I'
Specimen
4%
-
Excitation
light
-----+
Emitted light
Crossover
The spectral characteristics of the dichroic mirror when it is positioned
at an inclination of 45" to the optical axis of incident light is shown in
Fig. 2. Because a cross-over exists between transmittance
and reflect-
ance, it is necessary to use an appropirate combination of exciter and
barner filters in conjunction with the dichroic mirror. This is necessary
to achieve a good contrast image through fluorochrome
excitation in
the specimen, at the desired wavelength.
When the dichroic mirror is inclined 45" to the optical axis of incident
light, it reflects the excitation light towards the objective, and passes
unwanted wavelengths.
When the specimen is irradiated by the excitation wavelength,
it emits
a visible longer wavelength
corresponding
to Stoke's law. The barrier
filter mounted between the objective and eyepiece blocks out unwanted
wavelengths
providing a black background.
1
0