Olympus CHD Manual de instrucciones - Página 14

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Use of Immersion Objectives
1) To utilize the full numerical aperture of an immersion objective (with inscription "oil"). the objective and
specimen are immersed in an immersion oil in a following manner:
2) Focus on the specimen with a low power objective.
3) Put a drop of immersion oil on the specimen slide and the front lens of the immersion objective.
4) Turn the nosepiece to bring the immersion objective into the light path, and focus with the fine adjustment
knobs.
*
Use of the pre·focusing lever facilitates steps 2) - 4) above.
*
Care should be taken to prevent oil bubble from forming in the oil film; if any, swing the nosepiece to the
right and left reciprocally several times and re-apply immersion oil, since these bubbles greatly deteriorate
the lens performance.
*
Be careful not to stain other objectives with immersion oil, and after use, carefully wipe off the immersion
oil on the objective, etc. completely.
6
OPTICAL DATA
Objective
Type
E
0
,;A.chromat
Magnification
4X
lOX
40X
100X*
N.A.
0.10
0.25
0.65
1.25
W.o. (mm)
29.00
6.30
0.53
0.20
Focal length (mm)
31.05
16.45
4.59
1.90
Resolving power
(~)**
3.4
1.3
0.52
0.26
Eyepiece
Remarks
Spring-loaded
Total magnification
40X
100X
400X
1000X
CWHK10X
Focal depth
(~)
172.5
27.60
3.03
0.67
(Field number
181
Field of view (mm)
4.5
1.8
0.45
0.18
*lmmersion objective
**The resolving power is obtained with the fully opened aperture diaphragm.
o
"'0
-l
(')
»
r
o
~
»
The distance from the specimen or cover glass to the nearest point of the objec-
tive.
Resolving power:
The N.A. represents a performance number which could be compared to the
relative aperture (f-number) of a camera lens. The quantity of light which the
objective receives from the object increases with the square of the performance
number.
The resolving power of a lens is measured by its ability to separate two points.
The distance between the upper and lower limits of sharpness in the image
formed by an optical system. As you stop down the aperture iris diaphragm,
the focal depth becomes deeper.
The larger the N.A. of the objective the
shallower the focal depth.
A number that represents the diameter in mm of the image of the field dia-
phragm that is formed by lens in front of it.
Field-of-view diameter:
The actual size of the field of view in mm.
Field number:
Focal depth:
Numerical aperture:
Glossary:
Working distance:
Total magnification:
Objective magnfication x Eyepiece magnification.
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