Olympus CK2-BIP Manual de instrucciones - Página 10
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2
Phase Contrast Observation
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Legend comes atop.
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Legend comes atop,
Fig 15
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Fig 18
D
Pre aration of the philSe slider
Put the li!tlt annuli for 4X
ill
and 40X
<V
objectives inlO the phase
slider. The annulus@ for lOX is also use<! for 20X objectives. The
annuli for 4X and lOX {lOX
I
objectives are centered by means of
the centering lever@, (Fig.
15)
Mount the light annulus
<V
for 40X objective into the center hole
of the phase slider (as the ARROW indicates).
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Insert the phase slider
<D
into the slot in the condenser in such a
manner that its engraved surface with figures faces upward. and
the notched side toward the lamp housing pillar. Rotate the aper-
ture iris diaphragm lever
<V
all the way to the left, and insen the
green filter provided intO the filter mount (j). (Fig. 16)
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Cantratl
0 tha ;
t
u
1) Place a specimen on the
st~
and focus on it.
Remove one of the eyepieces from the observation tube and insert
the centering telescope CT·5
CD
provided into the observation
tube. {Fig. 17)
Ascertain that objective magnification and annular ring are matched.
Rotate the top lens assembly of the centering telescope to bring
the bright ring (light annulus) and the dark ring (phase annulus)
into focus.
Operate the centering lever of the light annulus until both annuli
are concentric and superimposed. (Fig. 181
*
If a specimen slide or the bottom surface of I cufture ve$Sill is
not flat. either annulus is likely to slip off the center while
centering the other. In such a caSIl. repeat centering adjustment
again. This trouble, however, rarefy occurs with hi!tler magnifica·
tions, so that the 40X philSe annulus needs no centering adjust·
ment.
21 After centering adjustment with the lOX objective, check the
centration with the 20X objective.
3} After centration is complete, remove the CT.s and insert the eye·
piece back into the observation tube.
Now it is ready for phase contrast observation.