Olympus FV1200 MPE Manual del usuario - Página 6
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Olympus FV1200 User guide
4. After observation, click
F. Image Acquisition (Multicolor imaging)
1. Click on Dye list
dyes you want to observe from the list. Click Apply button (if the Assign Dye Manually check box is
checked, uncheck it before pressing Apply button). It will activate the laser lines and the detection
channels according to the dyes you selected.
If the Selected dyes upon opening the program are not ones that you want to use, click the All Clear button
first before selecting your dyes.
2. Choose Image size (default- 512x512) from Size panel
3. Press
button (if not pressed) to automatically adjust HV and Offset values according to the scan
speed.
scan speed (around 20 us/pixel).
4. Click
button to scan the sample. It will scan fast at 2 us/pixel and show low-quality (pixelated)
image in a Live View window. While scanning, focus onto the region of interest with the remote focus
controller or by clicking arrowhead buttons (
5. Click Stop button
or [DIA] button on the TPC to close the shutter.
button on the Image Acquisition Control Window and double click on the fluorescent
to stop scanning.
. Slide the scan speed knob to select the image
) in the Microscope window.
June 23, 2015
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