Olympus FV3000 Manual de instrucciones - Página 6

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XY Image Acquisition(1)

②

⑩

④

①

⑧

Reducing noises by

Averaging.

Adjusting Confocal

Aperture.

⑨

Laser Intensity

Sensitivity

⑤

③

⑦

① Select "VBF" in the [PMT Setting] Tool Window.

Assigning the detector to channel

② Press the

Tool Window to open the [Dye & Detector Select]

dialog box.

③ Press the

Dye.

④ Double-click the name of the fluorescence dye to

observe.

Virtual Channel Scan

This mode allows to acquire multiple CH image

whose channel number is larger than the

number of detector.

⑤ When registering a fluorescence dye, TD channel

is registered automatically. If you don't need,

double-click the TD channel to cancel it.

⑥ If you want to observe with multiple phases using

the virtual channel scan, press the

to add the phase. And drag&drop the selected

"Dye" to the observation channel list of the phase

you want to add.

⑦ After setting all channels, press the [OK] button.

Adjusting the live image

⑧ If you want to acquire multiple channel image,

select Sequential scan "Line" in the [PMT Setting]

Tool Window.

⑨ Press the

button on the [Live] window.

Adjust focus and set Laser Intensity(%),

Sensitivity(HV), Gain and Offset on [PMT setting]

Tool Window.

⑩ If you use virtual channel scan, press

to switch phases. On both phases, adjust focus

and set Laser Intensity(%), Sensitivity(HV), Gain

and Offset.

【Hi-Lo Mode】

pixels of intensity 4095 are shown in red.

Pixels of intensity 0 are shown in red.

button on [PMT Setting]

button to reset the Assigned

button