Olympus IX81 Folleto y especificaciones - Página 7

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Nomarski DIC system offers the choice of optimal resolution or
high contrast in live cell observation.
DIC
DIC

Differential Interference Contrast

Live cells specimens vary in thickness from from that of a nematode
worm such as C. elegans to a monolayer of cultured cells. The
requirements for DIC are also varied according to the specimen
from thinner cells being almost invisible to thicker specimens having
a lot of inherent contrast. Olympus provides three DIC systems with
varying amounts of shear. Small shear, high resolution sets are
excellent for thicker specimens. High contrast prism with twice the
normal shear are excellent for very thin specimens.
Selecting the optimum DIC prism optimum for specimen
thickness and objective magnification
Thin specimen
(Big shearing value)
U-DICTHC
for superior contrast with thin specimen
observation
U-DICT, U-DICTS
for general observation
U-DICTHR
for superior resolution with thick specimen
observation
Thick specimen
(Small shearing value)
10X
40X
100X
DIC sliders
High resolution DIC slider for transmitted
High contrast DIC slider for transmitted
light/U-DICTHR
light/U-DICTHC
Comparison of thick specimen (C. elegans), showing differences in shearing value
DIC observation using U-DICTHR
Comparison of thin specimen, showing differences in shearing value
DIC observation using U-DICTHC
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Long working distance universal condenser/IX2-LWUCD
Combining a long working distance (27 mm) and a high numerical
aperture (N.A. 0.55), the LWUCD condenser accommodates most
incubation chambers and T-Flasks. The 5-position turret provides
versatility with DIC or phase inserts. DIC components are specially
designed to obtain high-contrast, high-resolution images with 20X
and 40X objectives.
• New DIC system gives a wider choice
UIS2 expands the selection of DIC
applicable objectives. Each condenser
prism is compatible with more lenses
making setup and configuration easier.
Shift DIC sliders for transmitted light/
U-DICTS
DIC sliders for transmitted light/U-DICT
HR/HC optical elements for IX2-LWUCD
and applicable objectives
DIC elements
IX2-DIC20HR UPLSAPO20X
IX2-DIC20HC UPLFLN20X
IX2-DIC40HR UPLSAPO40X2
IX2-DIC40HC UPLFLN40X
General type optical elements for
IX2-LWUCD and applicable objectives
DIC observation using U-DICT/ U-DICTS
DIC elements
IX2-DIC10
IX2-DIC20
IX2-DIC40
IX2-DIC60
IX2-DIC100
DIC observation using U-DICT/ U-DICTS
Water immersion DIC condenser/IX2-DICD
High performance DIC condenser designed
for excellent optical performance and
specimen access in high magnification
observations. Designed for specimen
access, all controls are front mounted
including prism exchange and aperture
control. Three high numerical aperture top
lenses are available including the water
immersion IX2-TLW that offers 0.9 N.A.
with 3.7 mm of working distance and a 40°
approach angle for micro manipulations.
Top lens combination
Numerical Aperture Working Distance
(N.A.)
(W.D.)
IX2-TLW
0.9
3.7 mm
U-TLD
0.9
1.5 mm
U-TLO
1.4
0.63 mm
Condenser adapter/IX-ADUCD
This is the condenser adapter for upright
microscope condensers on the IX2,
including the 8-position turret condenser
(U-UCD8-2) for maximum system flexibility.
This combination allows the use of various
optional element with high N.A., just
rotating the smooth turret for switching
them easily. The IX2 illumination pillar also
offers a 'condenser-only' tilt mechanism to
quickly allow access to the
specimen without tilting
the entire illumination pillar.
* IX2-TLW cannot be used for U-UCD8-2
Applicable objectives
LUCPLFLN20X
UPLFLN40XO
LUCPLFLN40X
Gliding stage/IX2-GS
Applicable objectives
The Gliding Stage was designed for quick
UPLSAPO10X2
rotation of the specimen using your
UPLFLN10X2
UPLSAPO20X
fingertips. With 20 mm of X-Y travel,
UPLFLN20X
360 degree rotation and completely flat
LUCPLFLN20X
UPLSAPO40X2
surface, a specimen such as the nematode
UPLFLN40X
worm C. elegans can be quickly brought into
UPLFLN40XO
LUCPLFLN40X
the correct position and alignment for
PLAPON60XO
injection or micromanipulations.
UPLFLN60X
UPLFLN60XOI
LUCPLFLN60X
UPLSAPO100XO
UPLFLN100XO
UPLFLN100XOI
Immersion
40°
Water
Oil
Water immersion DIC condenser IX2-DICD + water immersion
top lens IX2-TLW
IX2-GS
New DIC system
Simple principle of Nomarski DIC
microscopy
Nomarski DIC amplifies contrast by using the
phase difference which occurs when light
passes through material with different
refraction or thickness value (e.g. a cell) in a
particular medium (e.g. water). The wave
direction of light from the microscope light
source is unified in a polarizer (condenser
side); and when it passes through the
condenser side DIC prism, it separates into
two beams which cross each other at right
angles. The distance of separation is called
the shearing amount. When two such
separated beams pass through a medium
with different refraction values (e.g. a cell), one
of them is delayed; and when the two beams
are re-composed by DIC prism (the observa-
tion side) and pass through the analyzer, the
interference effect produces the contrast.
This is the principle of Nomarski DIC.
Polarizer
DIC prism
Condenser
Specimen
Shearing value
Objective
DIC prism
Analyzer
Olympus has developed the most suitable
DIC prisms for different types of specimen,
based on the shearing amount. When DIC
contrast is low, the specimen is hard to
observe, while high contrast also hinders
observation because of excessive glare.
Olympus has therefore developed three
different types of DIC prisms to ensure clear
observation for every kind of specimen.
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