Olympus CKX-RFA Manual de instrucciones - Página 19

Navegue en línea o descargue pdf Manual de instrucciones para Equipos de laboratorio Olympus CKX-RFA. Olympus CKX-RFA 36 páginas. Reflected fluorescence system

Imprimir página

Fig. 7

Fig. 8

Centering the Field Iris Diaphragm

1. Engage the 10X objective in the light path, push in the fluo-

rescent mirror switching knob to engage the B-excitation

mirror in the light path, and set the filter slider to the central

open position or the position of the ND filter.

2. Place a specimen on the stage and bring into approximate

focus.

3. Turn the field iris diaphragm lever 1 counterclockwise little

by little, and confirm the iris diaphragm position.

4. Using the provided Allen wrench, turn the two field iris

centering screws 2 alternately to move the image of the

diaphragm to the center.

5. After moving the diaphragm image to the center, open the

field iris diaphragm. As this makes slight deviation notice-

able, adjust the centering precisely.

6. After completion of centering, engage the iris diaphragm

diameter until it just circumscribes the field of view.

Adjusting the field iris diaphragm

The field iris diaphragm adjusts the diameter of the illumi-

nating beam to obtain good image contrast.

Keeping the field iris diaphragm stopped down to the small-

est required area for each observation makes it possible to

prevent color fading of areas outside the observation target

region.

According to the objective in use, adjust the diaphragm

image using the field iris diaphragm lever so that the

field of view is circumscribed by the field iris diaphragm

to exclude stray light.

REFLECTED FLUORESCENCE SYSTEM

FOR CKX41

(Figs. 7 & 8)