Olympus SZX-RFL3 Manual de instrucciones - Página 18

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1. Fluorescence Filter Combinations and Applications

Using the Dummy Filter

}The dummy filter sliders can be used in fluorescence observation by attaching a filter.

Dummy excitation filter slider

1. Remove the ring spring then the light shield.

2. Insert the required excitation filter (25 mm dia., 6 mm or

less thickness), and lock it with the ring spring.

Adjusting the Lighting Head (When the SZX-ZB9 Is Used)

}The angle of the lighting head should be adjusted when the DFPLAPO1X-2 objective is used.

Fig. 20

1. Place a specimen with an explicitly indicated center (for example a piece of

paper with a cross marking on the center), focus on the specimen at

higher magnification and bring the center (cross) to the center of the field

of view.

2. Rotate the filter turret to engage a filter, other than a GFP or U-excitation

filter, in the light path.

3. Loosen the rotation adjustment screw @ on the lighting head using the

Allen screwdriver, adjust the angle of the lighting head so that the cross

marking aligns with the center of illumination field, then tighten the screw.

4. Change the magnification from 10X to maximum as indicated on the

zooming knob to make sure that no vignetting occurs.

Dummy barrier filter slider

1. Insert the required barrier filter (20 mm dia., 6 mm or less

thickness).

2. As this slider does not use the ring spring for locking, it

can be used in the horizontal positioning.

To prevent the filter from dropping, it is recommended to

fix the filter on the slider by using a piece of adhesive

tape or a bonding agent.

SZX–RFL3