Nikon Live Cell 2 Manual - Página 10

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Multi-dimensional acquisitions

For timelapse, large images, Z-stacks and multipoint imaging, the ND acquisition window is used. This allows files to

be saved whilst image acquisition is running.

Timelapse imaging

1. For timelapse imaging, select the Time tab in ND

acquisition and add a time point into the ND

acquisition menu.

2. Set a frame interval and duration that are

suitable for your samples. From the drop-down

menu in each of these sections the time units can

be adjusted from msec to hours. The interval can

be set to 'no delay' which will begin a time loop

immediately after the previous. On the duration

box, the time can also be set to continuous which

will require the user to stop the acquisition after

the desired period.

3. Other ND acquisition modes can be added to the

timelapse (e.g. z-stacks, multipoints) which when

checked and set up in the ND acquisition window

are added to the image sequence. NOTE –

multidimensional parameters will affect the

achievable frame interval of the timelapse.

Clicking 'timing' will allow you to see if the

interval is set at an achievable rate.

4. To begin the time lapse image press 'Run Now'

Multi-points

1. To image multi-points, select the XY menu in ND

acquisition.

2. To add image coordinates click add and click the

arrow point at the coordinates to capture them.

Check the include z box to allow capture of z in

the same way (if PFS is used this can also be set

in the same way).

3. The arrows, shown in red boxes in Fig. 25, are

used to update stored positions.

Nikon Imaging Centre @ King's College London

Nikon

King's College London

Imaging Centre

Fig. 24

Fig. 25