AmScope B270 Manuel - Page 16

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2.3b Adjusting the Aperture

[image: aperture lever]

The condenser has a built-in iris diaphragm which creates an internal aperture. This aperture controls the amount of light

which passes through the condenser, and partially collimates the light. Decreasing the size of the aperture restricts the

amount of light used which in turn reduces the amount of scattered light. The notable effects are a reduction in bright-

ness as well as increased depth of focus. Decreasing the aperture too much will result in diffraction artifacts which dete-

riorate image quality. As objective magnification increases, more light will be requires to accommodate higher numerical

apertures. The aperture should be increased accordingly.

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