ATLAS BIOTECNOLOGIES AB10000 Manuel d'instruction - Page 5

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• If the desired volume is lower than set volume shown

by the counter, the operator should turn the pipetting

pushbutton (Fig. 1A2) or the black adjustment knob

(Fig. 1B) to the direction diminishing counter readings

to the required volume. Before achieving the required

volume slowly rotate the knob and observe carefully

diminishing reading to avoid accidentally passing the

setting value.

• If the desired volume is higher than set volume shown

by the counter, the operator should turn the pipetting

pushbutton (Fig. 1A2) or the black adjustment knob

(Fig. 1B) increasing the value until the lower figure

wheel comes 1/3 of a turn beyond the required setting

and then slowly backward until the setting reaches the

desired volume. Make sure not to pass the setting

value.

If the knob is accidentally turned too far, the process

must be repeated. The desired volume must always be

set from the higher value in the order of decreasing value.

3 - ASPIRATING AND DISPENSING LIQUIDS

Place a tip on the shaft of the pipette. See section 6 for

the appropriate tip. Press the tip on firmly using a slight

twisting motion to en sure a positive, airtight seal.

Important: Never aspirate liquids into the pipette

without a tip attached.

Aspiration

Press the pushbutton to the first positive stop, (Fig. 2A).

Holding the pipette vertically, immerse the tip into the

sample liquid. The depth to which the tip is immersed in

the sample liquid depends on the model.

Model

AB2

AB10

AB20, AB50, AB100

AB200, AB250, AB1000

AB5000

AB10000

Release the pushbutton slowly and smoothly to aspirate

the sample, (Fig. 2B). Wait one second and then with-

draw the tip from the liquid. When the pipette tip is

immersed not as deeply as the recommended depth or

Immersion depth (mm)

≤ 1

2 ÷ 3

3 ÷ 6

5 ÷ 7

when the pipetting pushbutton is rapidly released air

may enter the disposable tip.

Avoid touching the orifice of the tip.

Dispensing

• Place the end of the tip against the inside wall of the

vessel at an angle of 10 to 40 degrees.

• Press the pushbutton smoothly to the first stop, (Fig. 2C).

Wait one second.

• Press the pushbutton to the second stop to expel any

remaining liquid, (Fig. 2D).

• Keeping the pushbutton depressed to the very end,

remove the pipette by drawing the tip against the inside

surface of the receiving vessel.

• Release the pushbutton to its starting position, (Fig. 2E).

• Eject the tip by pressing the tip ejector button, (Fig. 2F).

Remember to change the tip whenever a different kind

of liquid is to be sampled.

4 - PRE-RINSING

When pipetting liquids of higher viscosity or lo wer surface

tension than water (e.g. sera or or ga nic solvents), a film

of liquid is formed on the inside wall of the pipette tip.

This film can create an error. Since the film remains rela-

tively constant in successive pipetting operations with

the same tip, this error can be avoided by forming the

film before transferring the first sample. This is done by

aspirating a sample and dispensing it back into the same

vessel. Since the film is already formed, all of the follow-

ing samples will have better accuracy and repeatability.

This pre-rinsing operation should be repeated when the

vo lume to be aspirated is changed or when a new tip is

used.

5 - DENSE AND VISCOUS LIQUIDS

The pipette specifications of accuracy and precision are

based on pipetting distilled water. The handling of liquids

with physical qualities of density, viscosity and surface

tension differing extremely from water may need a gravi-

metrically checked compensation of the volume setting.

Normally the degree of error resulting from heavy or vis-

cous liquids is negligible if the pipetting is done slowly

and carefully. It is most important to give the liquids

ENGLISH