Active Motif 53130 Manuel de l'utilisateur - Page 6

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Introduction

PIXUL Multi-Sample Sonicator

Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome, and whole

genome sequencing, require precise sample fragmentation (200-600 bp) for compatiblity with

short read sequencing platforms. Shearing by mechanical sonication, as opposed to enzymatic

methods, is preferred for its unbiased nature and ability to produce a relatively narrow frag-

mentation profile. However, the currently available sonication systems can be incompatible with

high-throughput sample processing. The PIXUL Multi-Sample Sonicator provides a flexible and

reproducible shearing profile for up to 96 samples processed in parallel.

Furthermore, high-throughput sonication on other platforms is currently limited by the cost of

consumables (tubes, plates, and reagents). However, the low cost, round-bottom plates (cat. no.

53139) used in the PIXUL instrument are compatible with cell culture and shearing, eliminating

sample transfer steps that are inconvenient and can lead to loss of sample. Furthermore, the PIXUL

Coupling Fluid does not require lengthy degassing and can be adequately circulated and ready for

sonication in approximately 15 minutes.

Sonication of the samples is quick and easy. Using the touchscreen user interface, researchers are

able to specify unique sonication parameters for each column of the 96-well plate, enabling pro-

cessing of many sample types for many different applications in the same microplate. Most runs

are complete within 10-30 minutes, ensuring that sample sonication will not be the bottleneck for

your high-throughput applications.