Major Science MJ-105-S Manuel d'instruction - Page 12
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with the flask swirling once every minute. The solution should be heated until
all crystals are dissolved. This is best viewed against a light background.
Crystals appear as translucent crystals. These will interfere with sample
migration if not completely dissolved.
Note: The gel must be cooled to between 50°C and 60°C degrees before
pouring.
3. Install the Gel Tray in the Gel Maker Stand.
4. Select the suitable Analytical Comb for the application.
5. Pour approx. 25 to 30 ml of agarose solution for a large tray; pour 12 to 15
ml for a small tray. The tray contains the agarose level for reference.
Note: Pour the agarose carefully so as not to generate bubbles. Any bubble
that surfaces could be smoothed to the edge of the gel and dispersed
using a pipette tip.
6. Place the comb(s) into the grooves.
7. As for the MJ-105 series, both Gel Maker Stands can be connected together
for user convenience.
Gel Running
1. Carefully remove the comb and transfer the gel, including the tray, to the
electrophoresis tank.
2. Refer to the molecular biology protocols for sample preparation.
3. Fill the electrophoresis tank with buffer until the gel is just filled to the top.
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