CORNING MicroDEN Manuel d'instruction - Page 5

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Method 1: Positive Pressure

1. Unclamp the pumps by pressing upwards at the dotted triangle on the

pump head.

2. Place the tubing onto the pump rollers. The colored tubing marker should

be to the left of each pump and match the colored flag next to each pump

(Figure 2).

3. Leave the pumps unclamped.

4. Connect a pipettor to the sterile filter of the inlet bottle and use the "pipette

down" button to pressurize the inlet bottle (Figure 3). Cell solution will flow

from the seeding bottle, through the tubing, and into the flasks.

5. Tilt the seeding bottle to allow all the liquid to flow into the dip tubes. This

ensures that all of the cell solution is perfused into the flasks.

6. Stop pipetting when the seeding bottle is empty. The inlet side tubing will

still be filled with cell solution.

7. If cell solution enters the outlet tubing, "pipet up" to reverse flow.

8. Clamp the tubing onto the pumps by pressing down on the pump head clamp.

9. The tubing holder on each side of the display may be used to help secure the

tubing in place.

NOTE: Corning® MicroDEN® flasks can be filled separately by clamping one of

the tubes in the pump to stop flow. Liquid will flow through the unclamped tube.

Thermoform

tray

Color

markers

match

Pumps shown

in clamped

position

Figure 2. Set up for cell seeding.

Outlet ports

Inlet ports

Function

button

Tubing

holder

ON/OFF

switch

Power cord

connection

Pipet

Figure 3. Pressurizing the inlet bottle.

Method 2: Pump Perfusion

1. Unclamp the pumps by pressing upwards at the dotted triangle on the pump

head.

2. Place the tubing onto the pump rollers. The colored tubing marker should

be to the left of each pump and match the colored flag next to each pump

(Figure 2).

3. Clamp the tubing onto the pumps by pressing down on the pump head

clamp.

4. Turn the Corning® MicroDEN® unit "ON," and select "Seed" using the function

button on the unit.

5. The pumps will perfuse the cell solution from the seeding bottle into the flasks.

6. Tilt the seeding bottle to allow all the liquid to aspirate into the dip tubes.

This ensures that all of the cell solution is perfused into the flasks.

7. Stop perfusion by pressing the MicroDEN unit function button when the

seeding bottle is empty. The inlet side tubing will still be filled with cell

solution.

NOTE: Corning MicroDEN flasks can be filled separately by unclamping one of

the tubes to stop flow. Liquid will flow through the clamped tube.

Helpful Seeding Tips

During seeding, tilt the inlet bottle to allow all the liquid to flow into the dip

◗ ◗

tubes. This ensures that all of the cell solution is perfused into the flasks.

Pipet up

or down

MPCs for bottle

change over