Olympus BX3-RFAS Manuel d'instructions - Page 25

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Olympus BX3-RFAS Manuel d'instructions
17. For red fluorescent protein (RFP)
18. For mCherry
U-FRFP
U-FMCHE
Wavelength (nm)
Wavelength (nm)
Typical Example of Emission Spectrum of Ultrahigh-Vacuum
Mercury Burner Spectrum
Wavelength (nm)
For fluorochrome emission, a light beam having a specific wavelength is selected from a
wide spectrum of wavelengths. The five major peaks of luminance are at wavelengths of
365/366, 404.7, 435, 546.1 and 577.0/579.1 nm. In addition, light beams having wavelengths
of 334.2 and 490 nm (at rather low luminance) are also applicable to fluorochrome
emission.
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