Olympus BX51M Manuel d'instructions - Page 13

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Fig. 10

Field iris diaphragm image

Field of view of eyepiece

Fig. 11

70%

30%

Aperture iris image

Centering the Field Iris Diaphragm (FS)

1. Slide the mirror selector lever @ to "BF".

2. Engage the 10X objective by rotating the revolving nosepiece, place the

specimen on the stage and adjust approximate focusing.

3. Pull out the FS knob ² on the reflected light illuminator to reduce the

aperture iris diaphragm a little.

4. Rotate the two FS centering screws ³ using the Allen screwdriver to

adjust so that the field iris image becomes concentric with the field of

view.

5. While pushing in the FS knob ², open the field iris diaphragm until the

field iris image inscribes the field of view. If the image is found to be

eccentric, adjust the centering again.

6. Open the field iris so that its image is almost the same size as (i.e. sub-

scribes) the field of view.

Using the Field Iris Diaphragm

· In reflected light brightfield observation

The field iris diaphragm adjusts the illuminated area to obtain an image

with high contrast.

According to the objective in use, adjust the FS knob ² of the reflected

light illuminator until the iris image circumscribes the field of view to

block unnecessary light.

· In reflected light darkfield observation

The field iris diaphragm must be opened by pushing in the FS knob ².

Centering the Aperture Iris Diaphragm (AS)

1. Slide the mirror selector lever @ to "BF".

2. Engage the 10X objective by rotating the revolving nosepiece, place the

specimen on the stage and adjust approximate focusing.

3. Remove the eyepiece, look into the eyepiece sleeve and pull the AS

knob ² so that the aperture is about 70%.

4. If the center of the iris diaphragm is deviated, center it by rotating the two

AS centering screws ³ using the Allen screwdriver.

Using the Aperture Iris Diaphragm

· In reflected light brightfield observation, optimum observation is gen-

erally possible by setting the aperture to between 70% and 80% of the

aperture number of the objective.

· In reflected light darkfield observation, the aperture must be fully opened

by pushing in the AS knob ².

}With some specimens, an image with high contrast and little flare may

sometimes be obtained when the aperture is slightly closed. It is there-

fore recommended to also try a slightly closed aperture.

BX51M

(Fig. 10)

(Fig. 11)