Olympus CHT Manuel d'instructions - Page 15

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Également pour Olympus CHT : Manuel d'utilisation (23 pages)

Olympus CHT Manuel d'instructions
Fie.
22
Exi l pupil
of objectille
(Image of aperture diaphragm as seen
through empty eyepiece tube)
Fig.
23
Field iris diaphragm
J
Fig. 24
Fie.
25
II
Adjustment of the a erture iris diaphragm
The opening of the aperture iris diaphragm built in the condenser
can be adjusted to match with the numerical ar::erture of the objec-
tive in use, in order to achieve optimum objective performance as
depth of focus, image cDlltr'ast and resolution.
1)
T urni ng the diaphragm lever
CD
counterclockwise reduces the d ia-
phragm or::ening. (Fig. 22)
2) Remove the eyepiece, and looking at the exit pupil of the Objective
through the empty eyer::iece tube, adjust the opening of the diaphragm.
Generally, i
I
is preferable to stop down the aperture diaphragm to 70%
1O
80% of the objective
NA
(Fig. 23)
1ft he specimen isl igh lIy stained, or al most colorless and tra nsparent,
further reduce the diaphragm or::ening to increase contrast for better
image observation. Be careful, ho'wever, if the diaphragm is stopped
down too much, the resolution will be deteriorated.
II
Adjustment flf
the field iril-diaphragm (for 'CH2-F-S only'
') The field iris diaphragm controls the diameter of the ray bundle im-
pinging on the specimen and therefore, by stopping down the field
diaphragm until it is slightly larger than the field of vi
e'.'J ,
it can reduce
stray light, which in turn increases image definition and contrast. (Fig.
24)
2 } Turning the field d'laphragm ring
CD
{Fig. 19} counterclockwise reduces
the diaphragm opening, or reverse the ring to increase the opening.
1m
Tension adjustment of the coarse adjustment knobs
') A tension adjustment ring
(J~
is provided next
to
the coarse adjustment
knob. With this device the tension of the coarse adjustment is freely ad-
justable for either heavy or light movement, depending upon operator
preference. (Fig. 25)
Applying the tip of a large screwdriver at a groove in the periphery of
the
tension adjustment ring
CD,
rotate the ring in the direction of the
arrow to increase the tension, or reverse the ring to loosen.
2) However, do not Icosen the tension adjustment ring too much, because
this may cause the stage
1O
drop or the fine adjustment knobs to slip.
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