Olympus IX70 Manuel d'utilisation - Page 26

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values are stored until someone alters them. If we have problems with users altering main definitions, MF

can be locked into Administrator/multi-user mode to prevent this.

Now you may want to setup the experiment details using Cfg Expt sidemenu or main Configure

(open it using top sidemenu button if missing).

Hit 'start focusing' to activate

camera (or toggle shutter to find cells by

eye). Note you can change binning and

exposures in this dialog (in case you

guessed wrong), test the stored values, or

store the current values into the 'cfg acq'

settings with buttons here. Check other

wavelengths by clicking on the list

entries. Note subarray controls.

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Refer to the MM section on filter definitions

currently used. Create a new name, select correct filter

cube position from list. This example is not correct

since the Sutter Lambda wheel positions are not

defined (and must be). Wheel B is the emission wheel,

Wheel A is the excitation wheel. If this was 380 setting

for Fura-2, Wheel A should be checked and set to

380/nd1 filter. Wheel B should be set to OPEN.

(confused? Ask for help!) Finally, set the Lambda

shutter A to 'active' (open only for actual image

collection). Now hit Add/replace. Once defined, the

menu. You can set the collection intervals per

wavelength, save intervals and screen update

intervals. To run fast, you might display (a slow

step) every 3 image. To run even faster (takes about

50msec to change filter positions) you can collect

one of the wavelengths (like 340 for Fura-2) every

other or every ten F380 images (software uses old

value until refreshed). Highest speeds require

streaming (no display at all), short exposures (will

limit rate) and locking the shutter open (see config

acquisition, config illumination)

Run the experiment using the Control menu

You probably must use the Focus routine to turn on

illumination, find cells by eye,

, then hit Start Focusing.

PORT

PLEASE create versions vs replace!

MANUALLY SWITCH TO CAMERA